Alpha-Synuclein: Methods and Protocols
Hardcover 301 pages English Feb 18, 2019
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This volume provides the reader with detailed protocols that describe a variety of in vitro and in vivo techniques that study reductionist systems and human samples. The methods covered in this book explore a broad selection of topics such as functional aspects of alpha-synuclein biology, its lipid-interactions and misfolding, and functional deficits in cells and mice. Chapters also cover topics such as kinetic measurements of endocytosis and exocytosis in cultured neurons; electrochemiluminescence-based detection; yeast-based screens to target alpha-synuclein toxicity; mass-spectrometry of alpha-synuclein in rat cortical neurons; and expression and purification of untagged a-synuclein. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Alpha-Synuclein: Methods and Protocols is a valuable reference guide that aids researchers working on topics related to alpha-synuclein and its various aspects.
- Author:
- Tim Bartels
- Publisher:
- Humana
- Publication Date:
- Feb 18, 2019
- Number of pages:
- 301 pages
- Language:
- English
- Binding:
- Hardcover
- ISBN-10:
- 149399123X
- ISBN-13:
- 9781493991235