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Lipid Rafts: Methods and Protocols (Paperback)

by Erhard Bieberich

Humana

Paperback/Science 364 pages English Aug 23, 2021

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This volume gives a comprehensive insight into established and novel methods to analyze the structure and function of lipid rafts. This book covers topics such as isolation of lipid rafts and their functional analysis using biochemical methods; visualization of lipid rafts and their interaction with proteins using fluorescence-related methods; preparation of giant lipid vesicles and fluorescence spectroscopy; FRET and FRAP; and using photo-activated cross-linking of a ceramide analog combined with proximity ligation assay. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, Lipid Rafts: Methods and Protocols is a valuable resource for both novice and expert researchers interested in learning moreabout the function of lipid rafts in many areas of cell biology and medicine.
Author:
Erhard Bieberich
Publisher:
Humana
Publication Date:
Aug 23, 2021
Number of pages:
364 pages
Language:
English
Binding:
Paperback/Science
ISBN-10:
1071608169
ISBN-13:
9781071608166